Facilities Equipment

ICDT

Institute/Molecular Genetics & Neuroscience Lab

1) Histology and Imaging:

• Water Bath (PN-III-P4-PCE-2021-0333)

• Cryostat (UnitBv)

• Vibratome (UnitBv)

• Zeiss ImagerZ2 with Apotome

• Confocal (UnitBv)

2) Molecular Biology:

• -80 C Freezer (UnitBv)

• Liquid Nytrogen Storage Tank (UnitBv)

• Bacterial Incubator (PN-III-P4-PCE-2021-0333)

• Bacterial Liquid Culture Shaker (UnitBv)

• Preparative/Ultracentrifuge (UnitBv)

• Agarose and Polyacrylamide Electrophoresis, SemiDry transfer (UnitBv)

• PCR machines (UnitBv)

• Slide Hybridization Incubator (PN-III-P4-PCE-2021-0333)

• Rotating/Shaking Incubator (PN-III-P4-PCE-2021-0333)

• Quantus Fluorometer for DNA/RNA detection (UnitBv)

3) Cell Culture:

• Dedicated room (UnitBv)

• Cell culture incubator (UnitBv)

• Cell culture Hood (UnitBv)

• Inverted Microscope (UnitBv)

• Water Bath (PN-III-P4-PCE-2021-0333)

• Centrifuge (UnitBv)

• Refrigerator (UnitBv)

BEAM

Small Animal Experimental Facility

1) Animal Housing:

Two holding rooms with 1000 cages maximal capacity (currently 140 cages), with air conditioning (temperature, humidity, and air filtration to E11 grade), automated 12:12 light:dark cycle, individually ventilated cages with HEPA filtration (UnitBv).

2) Cage Wash Facility and Waste Disposal:

Water treatment (Deionized, Reverse Osmozis); Automated Cage Washer; Autoclave, Waste Disposal Incinerator (outside the building) (UnitBv).

3) Clean Materials Storage Room
4) Procedures Room:

Anesthesia (Isoflurane or CO2) equipment (UnitBv), Dissecting scope with red and infrared light sources (NEI_MOU), Computer-Guided Stereotactic injection apparatus (UnitBv), Microinjection pump (UnitBv), Capillary needle puller (PN-III-P4-PCE-2021-0333).

5) Behavior Analysis Room:

Apparatus for Optomotor and Optokinetic assays in mice (NEI_MOU), Pupillary Light Reflex (NEI_MOU), Visually Evoked Defensive Behaviors (NEI_MOU).

6) Histology Experiments area.
7) Electrophysiology Suite:

For multielectrode array recordings, using MEA-60 and 4000 "pixel" CMOS arrays, with visual stimulus presentation, Faraday Cage and perfusion systems (NEI_MOU).